Regulation of mitotic chromosomes via liquid-liquid phase separation between RNAs and condensin I
Mitotic chromosomes are composed of approximately 4,500 proteins and over 1,000 non-coding RNAs. We found that chromosome-associated RNAs were involved in the chromosomal association of condensin I and structure of mitotic chromosomes. Our results also suggested that the localization of condensin I on mitotic chromosomes was promoted by the liquid-liquid phase separation (LLPS) of condensin I promoted by RNAs. In this study, we plan to identify RNAs that promote LLPS of condensin I, and to elucidate how they regulate condensin I and the organization of mitotic chromosomes via LLPS
Back to Research Projects (FY2021-2022)
Keiji Kimura
Project Leader